Biochemical and Biophysical Characterization of Photosystem I from Phytoene Desaturase and ζ-Carotene Desaturase Deletion Mutants of Synechocystis Sp. PCC 6803
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چکیده
منابع مشابه
/ 1 - Carotene Hydroxylase Gene from PCC 6803 theCyanobacterium Synechocystis sp .
Kazumori Masamoto i, Norihiko Misawa2, Takakazu Kaneko 3, Reiko Kikuno4 and Hiroyuki Toh 4 i BiologicalLaberatot:y. Fkecutty ofEducation, Kumameto Uhiversity. Kumamote, 860 Jopan 2 CentratLaboratoriesforKby Tleehnoiogy. Kirin Brewenyy Co., Ltd., i-f3-5 flikuura. Kanazawa-ku, Ybkohama, Kanagawa, 236 Japan3 Kazusa DNA Research Institute, J532-3 Yana, Kisarazu, Chiba, 292 Jopan 4 Biomolecular Engi...
متن کاملNet light-induced oxygen evolution in photosystem I deletion mutants of the cyanobacterium Synechocystis sp. PCC 6803.
Oxygenic photosynthesis in cyanobacteria, algae, and plants requires photosystem II (PSII) to extract electrons from H(2)O and depends on photosystem I (PSI) to reduce NADP(+). Here we demonstrate that mixotrophically-grown mutants of the cyanobacterium Synechocystis sp. PCC 6803 that lack PSI (ΔPSI) are capable of net light-induced O(2) evolution in vivo. The net light-induced O(2) evolution r...
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Carotene isomerase mutant (crtH mutant) cells of Synechocystis sp. PCC 6803 can accumulate beta-carotene under light conditions. However, the mutant cells grown under a light-activated heterotrophic growth condition contained detectable levels of neither beta-carotene nor D1 protein of the photosystem (PS) II reaction center, and no oxygen-evolving activity of PSII was detected. beta-Carotene a...
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Carotenoids are isoprenyl pigments necessary for protection of the photosynthetic apparatus in a11 green plants. One of the intermediate steps in carotenoid biosynthesis is the conversion of phytoene to {-carotene via two dehydrogenation reactions catalyzed by the enzyme PDS. We have previously cloned cDNAs for this enzyme from soybean (Glycine max; Bartley et al., 1991) and tomato (Lycopersico...
متن کاملCloning and Characterization of the ζ-Carotene Desaturase Gene from Chlorella protothecoides CS-41
To elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS-41, the ζ-carotene desaturase gene (zds) was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends. The full-length cDNA sequence was 2031 bp and contained 1755 bp putative open reading frame which encodes a 584 amino acid deduced polypeptide whose compu...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2005
ISSN: 0021-9258
DOI: 10.1074/jbc.m500809200